Coding

Part:BBa_K2993003:Design

Designed by: Anass Fendrhoussi   Group: iGEM19_Rotterdam_HR   (2019-10-12)


Fusion protein of ARRAY2-GSAT-C-TEV-6xHisTag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 672
    Illegal SapI.rc site found at 982


Design Notes

remember that expression of zincfingerproteins is different than the usual expression of proteins, due to the zinc ions (Zn2+) that play a role in keeping the zincfinger in its native shape.



Source

The source of the split-TEV is the iGEM Munich team 2013. the GSAT linker was obtained from part number BBa_K1159102. This part has been E.coli optimized by IDT sequence software when the purchase order was made. it also contains a RBS

References